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Scenario 2: You need a lower concentration for smaller, more precise doses

The mobile phase comprised of (A) 10 mM ammonium formate in 60% aqueous acetonitrile containing 0.1% formic acid and (B) 10 mM ammonium formate in 9:1 mixture of isopropanol and acetonitrile with a 0.5 mL/min flow rate maintained during a 20 min run

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For the apo state, a final set of 216,118 particles was subjected to 3D homogeneous refinement, followed by local CTF refinement